ANTIBACTERIAL ACTIVITY OF INDIAN-ALMOND (Terminalia catappa) LEAF EXTRACT AGAINST Aeromonas hydrophila

The aim of this study was to determine the effects of various concentration of Terminalia catappa brown leaves extract which can inhibit the growth of Aeromonas hydrophila Stainier BIOTECH 10089, IFO 3820 isolated from Snakehead (Ophiocephalus striatus), locally known as Dalag. Qualitative phytochemical content of 7: caluppa was analyzed using the crushed- brown leaves that was extracted using 95% ethanol, filtered, and evaporated. The dried extracts were also dissolved in distilled water and serial dilutions were prepared to final concentrations of 25%, 50%, 75% and 100%. Chloramphenicol and distilled water were used as positive and negative controls, respectively. Antibacterial activity of every different concentrations was evaluated by disk diffusion method. The 7. catappa extract exhibited a mean zone of inhibition of 13.33 millimeters at 25%, 16.00 millimeters at 50%, 17.67 millimeters at 75%, and 19.67 millimeters at 100%. The positive control showed a mean zone of inhibition of 15.67 millimeters, while the negative control have no inhibition against the pathogen. The higher concentration of extract, the wider the inhibition zone diameter. The Minimal Inhibitory Concentration (MIC) of the extract required to inhibit the A. Hydrophilia was obtained at 7.82 microgram/millimeter. The results obtained shows that 7: catappa leaves extract exhibit phytochemical constituents such as alkaloids, steroids, saponins, and tannins, which may be responsible for the antimicrobial activities of the plant.