Somatic Embryogenesis and Plantlet Regeneration from Cotyledonary Tissue of Cashew (Anacardium occidentale L.)

Cotyledonary tissue was used as an explant and cultured on solid and semi- solid medium with different concentrations of BA and NAA as follows: To Knudson C (KC medium only) T1 (0.5mg / L NAA and BA), T2 ( 1mg / L BA and NAA), T3( 2mg / L BA and NAA). Somatic embryogenesis and plantlet regeneration were successful on solid KC medium with 2.0 mg/L BA and NAA while semi-solid medium with 1ma / L BA and NAA producing more somatic embryos developing into plantlets. However, statistical analysis using two-way Analysis of Variance (ANOVA) revealed that the induction and formation of somatic embryos and plantlet regeneration were insignificantly enhanced by the varying concentrations of BA and NAA as well as the different culture media used in the study.